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Water Use In Cryobiology

Deep freezing provides indefinite preservation of living tissue much like blood storage preserves blood cells. In cryobiology tissues are washed in an isotonic solution made with ultrapure water. They are frozen slowly and stored in liquid nitrogen.Tissue stored this way include skin cornea bone marrow blood vessels and human tumors.


Preservation through freezing has become more commonplace in the last 10 years. It was necessitated by the need for genetic stability in cancer research.The freezing preparation involves immersing fragments of tissue in glass ampoules containing a nutrient medium and glycerol in ultrapure water. After this the ampoules are sealed and cooled at a rate of 1oC per minute from ambient room temperature to -25oC. Then they are rapidly cooled to -70oC. This prevents ice crystal growth. Next they are stoed in liquid nitrogen at -195oC. When the tissue is ready to be used the ampoules are thawed rapidly using a water bath at  37oC. The tissue is then rinsed several times in a saline solution made with biopure water to remove the glycerol before being used.


Preserving tumor tissue for cancer research using this method does not alter a tumors morphology transplantablity or sensitivity to treatment with known growth inhibitors. In the first transplant generation thawed tumors grow more slowly than non forzen tumors. After several generations they will recover their normal growth rate. Lastly there is no measurable difference in viability between frozen and non frozen tumors.

Primer: Water Use In Research And Medicine
  • Water The Cleansing Agent
  • Water Use In Tissue Cell Culture
  • Water Usage In Pharmaceuticals
  • Pyrogens In Research And Medicine
  • Water Use In Blood Fractionation
  • Water Use In Cryobiology
  • General Use of Water in Research and Medicine


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    Lab Water Deionizer




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    APS Polaris D2 Water System with Quality Meter
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    This dual housing is designed to provide 1 g.p.m. of ultrapure water exceeding ASTM and CAP Type II specifications.  

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    PROUDLY MADE IN THE USA

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    APS Polaris D3 Water System with Quality Meter
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